Tissue culture propagation began in 1902 by Gottlieb Haberland, an Austrian botanist, who proposed the idea that plants could be propagated by cell isolation. Since then, the technology of plant propagation has been researched by many and has been the leading technology to produce a large amount of crop within a short amount of time when compared to traditional methods.
Tissue culture is the process of plant propagation in a steril environment that is able to culture a multiple of a single cell or plant without changing its character or gene.
In the recent years, there have been a growing number of research and studies on tissue culture propagation for aquatic plants species that are either endagered, commercialized or used for medicinal purposes.
Aqua Venture is also contributing to this growing concern for commercializing of aquatic plant species and preserving endangered species of aquatic plants. Our state-of-the-art laboratory has the capability of producing over 5,000 plants weekly, which also includes a R&D team that continues to develop efficient procedures for a wide variety of plants.
Everything in the tissue culture laboratory is conducted in a sterile manner. To have a successful culture, the plant must be free of all contaminates such as bacteria and fungi. Since the medium used to propagate the plants also attract contaminates, all measures to sterilize the tools and equipment is required. The basic steps required for a successful culture is as follows:
1. Determine which part of the plant will be used as an explant sample.
2. Wash the plant with clean water thoroughlyuntil clean.
3. Cut all leaves and branches off the explant.
4. Use different chemical solutions to sterilize the explant.
5. Place the explant in the agar medium and let it grown under growing lights.
Even thou, it may not seem diffficultform the following steps, the challenge is finding the correct chemicals to use, the duration use with the chemicals, and the amount of time it takes to sterilize the explant. Using too much or too long of time can damage the plant's cells, which could inhibit growth.
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